Figure 6.

AS160-4P blocks the effect of insulin on the intracellular HA-GLUT4-GFP. Ablation experiment with HA-GLUT4-GFP, IRAP-TR and the TR in the basal and insulin-stimulated state with or without expressing AS160-4P. The fraction in the specialized compartment is a measure of the amount of intracellular HA-GLUT4-GFP segregated away from the TR containing general endosomes. The data are the means ± SEM of five experiments. The data from the individual experiments were normalized to the Cy3/GFP ratio in control cells (no HRP-Tf uptake) for that experiment and the fraction in the specialized compartment is determined as described above. ^*p = 0.01; ^**p = 0.3.