Figure 3.

C. neoformans conjugation tube formation. Wild-type α and a cells were pre-incubated in Alexa Fluor 594 ConA and Alexa Fluor 488 ConA, respectively, to distinguish between α and a cells during conjugation. Cells were mixed, incubated on V8 medium for 4 h, and counterstained with filipin to identify sites of conjugation between cells (arrows). Cells were observed with Texas Red (Alexa Fluor 594), fluorescein isothiocyanate (Alexa Fluor 488), and DAPI (filipin) filter sets and the images were merged. Bar, 5 μm.