Figure 7.

Localization of actin in C. neoformans mating filaments. Glass slide mounts of wild-type mating filaments were costained with rhodamine-conjugated phalloidin and DAPI to visualize actin and nuclear dynamics during filamentous growth. (A) Actin patches are polarized to the tip of the growing dikaryotic filament and to the tip of the clamp cell (arrowheads). (B) Actin rings form at the site of septation during nuclear division. Nuclear migration has already occurred, separating one pair of duplicated nuclei (arrows) on either side of the actin ring, whereas one nucleus of the second duplicated pair prepares to migrate into the clamp cell (asterisk). (C) Actin rings also form at the clamp cell to separate the nucleus in the clamp cell from the terminal filament. Also depicted here is nuclear migration into the basidium (asterisk).