Table 1.
Strains and plasmids
| Strain | Genotype | Source/Reference |
|---|---|---|
| Serotype A | ||
| CSB1 | MATα pak1::URA5 ura5 | Wang et al., 2002 |
| CSB51 | MATα pak1::ura5 ura5 | Wang et al., 2002 |
| F99 | MATα ura5 | Wang et al., 2001 |
| H99 | MATα | Perfect et al., 1980 |
| JF99 | MATa ura5 | This study |
| JF219 | MATa ste20a::NAT ura5 | This study |
| JF265 | MATa pak1::NAT | This study |
| JF267 | MATa pak1::NAT ura5 | This study |
| KN99a | MATa | Nielsen et al., 2003 |
| PPW91 | MATα ste20α::URA5 ura5 | Wang et al., 2002 |
| PPW96 | MATα ste20α::ura5 ura5 | Wang et al., 2002 |
| Serotype D | ||
| CSB5 | MATa ste20a::ADE2 ade2 | Wang et al., 2002 |
| CSB8 | MATα pak1::URA5 ura5 ade2 | Wang et al., 2002 |
| CSB9 | MATα pak1::URA5 ura5 | Wang et al., 2002 |
| CSB10 | MATa pak1::URA5 ura5 | Wang et al., 2002 |
| CSB11 | MATα ste20α::URA5 ura5 ade2 | Wang et al., 2002 |
| KLB156-1 | MATa ste20a::ADE2 ura5 ade2 | Wang et al., 2002 |
| CSB15 | MATα ste20α::URA5 ura5 | Wang et al., 2002 |
| CSB21 | MATa ste20a::ADE2 ura5 ade2 lys1 | Wang et al., 2002 |
| CSB23 | MATα pak1::ura5 ura5 ade2 | Wang et al., 2002 |
| CSB25 | MATα pak1::ura5 ura5 | This study |
| CSB27 | MATa pak1::URA5 ura5 lys1 | This study |
| CSB48 | MATα ste20α::ura5 ura5 | Wang et al., 2002 |
| RDC5 | MATα cpk1::ADE2 ade2 | R. Davidson |
| JEC20 | MATa | J. Edman |
| JEC21 | MATα | J. Edman |
| JEC31 | MATa lys2 | J. Edman |
| JEC34 | MATa ura5 | J. Edman |
| JEC43 | MATα ura5 | J. Edman |
| JEC50 | MATα ade2 | J. Edman |
| JEC53 | MATa ura5 lys1 | J. Edman |
| JEC155 | MATα ura5 ade2 | J. Edman |
| Plasmids | ||
| pRCD83 | C. neoformans vector | R. Davidson |
| pCBN12 | serotype A PGPD1-STE20α | This study |
| pCBN19 | serotype D PGPD1-STE20α | This study |
| pCBN20 | serotype D PGPD1-STE20a | This study |
| pCBN23 | serotype D PGPD1-PAK1 | This study |
| pCBN34 | serotype A PGPD1-PAK1 | This study |
| pCBN47 | serotype A PGPD1-STE20a | This study |