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. 2004 Oct;15(10):4532–4543. doi: 10.1091/mbc.E04-04-0348

Figure 6.

Figure 6.

Overexpression of Pur proteins in AKR-2B fibroblasts attenuates SMA enhancer and full-length promoter activation by TGFβ1 or Smad proteins. (A) VSMP4 enhancer activation in transfected fibroblasts by TGFβ1, Smad2/3/4, or a combination of both was substantially repressed by Purα overexpression and fully repressed by Purβ. Compare lanes 2, 3, and 4 with lanes 5, 6, and 7 (Purα-mediated repression) or lanes 8, 9, and 10 (Purβ-mediated repression). (B) Smad-mediated activation of the full-length VSMP8 promoter in AKR-2B fibroblasts was substantially repressed by Purα and fully so by Purβ. Compare lane 5 with either lane 6 (Purα-mediated repression) or lane 7 (Purβ-mediated repression). The VSMP8 promoter normally is transcriptionally silent in fibroblasts but activated by TGFβ1 or Smad signaling intermediary proteins (compare lanes 1 and 5). For A and B, the various treatments and/or combinations of expression plasmids used for each transfection are shown at the bottom.