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. 2004 Oct;15(10):4735–4748. doi: 10.1091/mbc.E04-03-0225

Figure 5.

Figure 5.

Characterization of the tth-1 mutant. (A) Genomic structure of the tth-1 gene. Exons 1–3 are indicated by open boxes. The tth-1 (gk43) deletion is located in the upstream region of the gene. IR and IL indicate the location of PCR primers used in B. (B) Genomic DNA fragments of the tth-1 gene from a WT or tth-1 (gk43) homozygote were amplified by PCR using primers IR and IL. DNA size markers in kb are shown on the left. (C) Protein levels of tetraThymosinβ and actin in WT or tth-1 (gk43) homozygotes were examined by Western blot using anti-tetraThymosinβ and anti-actin antibody. (D) Reverse transcription-PCR of mRNAs for act-1 (1.1 kb), F08F1.3 (predicted size, 650 base pairs), and tth-1 (450 base pairs) in WT worms. DNA size markers in kb are shown on the left. (E) Morphology of WT or tth-1 (gk43) homozygous adult worms (scalebar, 0.2 mm). The tth-1 (gk43) homozygotes have a dumpy phenotype.