Figure 6.

Morphology and cytoskeletal organization of gonads from WT and tth-1-mutant adult. Dissected adult gonads (D, distal tip; Sp, spermatheca; O, oocytes) from a WT (A, C, and E) or tth-1 (gk43) homozygote (B, D, and F) were stained for actin (A and B) and tubulin (C and D) (scalebar, 50 μm). Merged images of actin (red) and tubulin (green) are shown in E and F. Actin is accumulated at the cortex of the oocytes (A, arrows) in WT but absent in the tth-1 mutant (B, arrows). The fine filamentous actin network observed in B is derived from the myoepithelial cells of the ovary, not from the oocytes. In the tth-1 mutant, deformed oocytes are found in the spermatheca (D and F, arrow).