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. 2016 Nov 29;8(11):2777–2789. doi: 10.18632/aging.101086

Figure 2.

Figure 2

(A) Quantitative real-time PCR analysis of hepatic mRNA expression of fgf21 and (B) quantitative analysis of plasma Fgf21 of ApoE−/− mice. Mice were fed either ad libitum (AL) or caloric-restricted (CR, 60% of ad libitum) for a short-term (4 weeks; n=14), mid-term (20 weeks; n=14) or long-term (64 weeks; n=14). At weeks 8, 16, 24, 32, 40, 48 and 64 plasma Fgf21 was measured. Signals were corrected to that of RPS18. Representative immunohistochemical images (C, original magnification x400) of Fgf21 accumulation in brain of long-term AL- (upper panel) and CR-fed ApoE−/− mice (lower panel) mice. Values are given as means ± SEM; ANOVA, post-hoc pairwise comparison tests.* p < 0.05 vs. AL.