Table 1. Related works on miRNAs and human aging samples, 2010-present.
| Study | Samples | Analysis method | Participants' ages when miRNA expression was assessed | Main findings * |
|---|---|---|---|---|
| Sredni et al. | Whole blood | Illumina 96-sample Universal Matrix Array (739 miRNAs) | 13 22-25 year olds (mean, 23.6 years) and 9 36-39 year olds (mean, 37.2 years) | The changes in global microRNA expression are associated with normal aging; the most differentially expressed microRNAs included miR-155, 8a, 142, 340, 363, 195, and 24 |
| Noren Hooten et al. | PBMCs | Multiplex qRT-PCR (over 800 miRNAs total) | 2 30 year olds and 2 64 year olds (male); validation in 14 young (mean, 30.1 years) and 14 old (mean, 64.2 years) individuals | 9 miRNAs downregulated (miR-24, 103, 107, 128, 130a, 155, 221, 496, 1538) |
| Gombar et al. | B cells | Deep sequencing (284 miRNAs); qRT-PCR (validation) | 3 63 year olds and 3 centenarians (female); validation in 27 individuals aged 50 to 100 years | 22 miRNAs upregulated, 2 downregulated; miR-363 downregulated (validation) |
| ElSharawy et al. | Whole blood | Microarray (863 miRNAs); qRT-PCR (7 miRNAs, validation) | 55 46 year olds and 15 centenarians and nonagenarians; validation in 17 younger (mean, 36.9 years) and 15 long-lived (mean, 101.5 years) individuals | 16 miRNAs upregulated, 64 downregulated; 3 miRNAs (miR-106a, 126, 30d) downregulated (validation) |
| Serna et al. | PBMCs | Microarray (1105 miRNAs) | 20 centenarians, 16 octogenarians, 14 young individuals | 6 miRNAs upregulated (centenarians vs. young) |
| Olivieri et al. | Plasma | ABI TaqMan miRNA PCR array (365 miRNAs); qRT-PCR (validation) | 11 20, 80, and 100 year olds; validation (only miR-21) in 111 healthy adults aged 20-105 (profiling cohort) and in 34 patients (mean, 87 years) with cardiovascular disease and 15 healthy centenarian offspring (mean, 72 years) | 46 miRNAs downregulated, 12 up- then downregulated, 5 upregulated in profiling cohort; one miRNA (miR-21) downregulated in validation cohort |
| Noren Hooten et al. | Serum | Deep sequencing and qRT-PCR | 20 young (mean, 30.1 years) and 20 old (mean, 64.2 years) individuals | 3 miRNAs downregulated (miR-151a-5p, miR-181a-5p and miR-1248) |
PBMCs, peripheral blood mononuclear cells.
*
The findings were made from the perspective of the long-lived participants, such as centenarians. There was no overlap in findings between all 7 studies.