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. Author manuscript; available in PMC: 2017 May 21.
Published in final edited form as: Nat Neurosci. 2016 Nov 21;20(1):42–51. doi: 10.1038/nn.4442

Figure 1. Regulation of feeding by ARCPOMC versus ARCVglut2 neurons.

Figure 1

a, Experimental schematic. b, d, e, Effect of CNO/hM3Dq stimulation of ARCPOMC neurons (b, d) or ARCVglut2 neurons (e) on dark-cycle feeding. c, Effect of CNO/hM3Dq stimulation of ARCPOMC neurons on iBAT temperature. f, Effect of CNO/hM4Di inhibition of ARCVglut2 neurons on light-cycle feeding. b–f, Data are presented as mean ± SEM. Mice from multiple litters. Repeated measures two-way ANOVA followed by Sidak’s multiple comparisons test (b, n = 5 animals; Treatment F(1,4) = 0.3265, P = 0.5983; time F(4,16) = 89.82, P < 0.0001; interaction F(4,16) = 3.037, P = 0.0485. 1h, P = 0.4831; 2h, P = 0.9980; 3h, P = 0.4831; 4h, P = 0.0610; c, n = 4 animals. Treatment F(1,3) = 8.931, P = 0.0582; time F(5,15) = 8.008, P = 0.0008; interaction F(5, 15) = 6.494, P = 0.0021. 30 min, ****P < 0.0001; 1h, ****P < 0.0001; 2h, **P = 0.0011; 3h, *P = 0.0102; 4h, ***P = 0.0008; d, n = 4 animals; Treatment F(1,3) = 0.7301, P = 0.4557; time F(4,12) = 75.69, P < 0.0001; interaction F(4, 12) = 1.475, P = 0.2703; e, n = 4 animals. Treatment F(1,3) = 63.28, P = 0.0041; time F(4,12) = 40.61, P < 0.0001; interaction F(4, 12) = 10.40, P = 0.0007. 1h, **P = 0.0040; 2h, ****P < 0.0001; 3h, ****P < 0.0001; 4h, ****P < 0.0001; f, n = 6 animals. Treatment F(1,5) = 16.55, P = 0.0096; time F(4,20) = 24.66, P < 0.0001; interaction F(4, 20) = 7.808, P = 0.0006. 1h, ***P = 0.0002; 2h, **P = 0.0016; 3h, ****P < 0.0001; 4h, ****P < 0.0001).