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. Author manuscript; available in PMC: 2017 May 21.
Published in final edited form as: Nat Neurosci. 2016 Nov 21;20(1):42–51. doi: 10.1038/nn.4442

Figure 5. Oxtr expression marks ARCGlutamatergic neurons that rapidly promote satiety.

Figure 5

a, Oxtr expression in ARCVglut2 neurons as determined by RNA-seq. b, Co-localization of POMC protein in tdTomato-positive ARCOxtr neurons. Approximately 50% of ARCOxtr neurons expressed POMC. c, Representative trace (top) and summary (bottom) of oxytocin’s effect on ARCOxtr neuron firing rate. Scale bars, 50 pA, 30 sec. 6 slices from 3 mice; mice from multiple litters; cells, n = 6. Baseline (M = 1.261, s.d. = 0.8192) versus Oxytocin (M = 2.991, s.d. = 1.495): Paired two-tailed t-test, t(5) = 4.253, **P = 0.0081. d, CRACM of ARCOxtr→PVH connectivity. Below, representative traces showing light-evoked EPSCs. e, Effect of CNO/hM3Dq stimulation of ARCOxtr neurons on dark-cycle feeding (n = 6 animals). f, Left, experimental schematic. Right, effect of ARCOxtr terminal photostimulation in the PVH on dark-cycle feeding (n = 5 animals). e, f, Data are presented as mean ± SEM. Repeated measures two-way ANOVA followed by Sidak’s multiple comparisons test. e, Mice from multiple litters, n = 6 animals. Treatment F(1,5) = 26.25, P = 0.0037; time F(4,20) = 63.88, P < 0.0001; interaction F(4, 20) = 15.31, P < 0.0001.1h, P = 0.1760; 2h, **P = 0.0025; 3h, ****P < 0.0001; 4h, ****P < 0.0001. f, Mice from multiple litters, n = 4 animals. Repeated measures two-way ANOVA: Treatment F(1,3) = 36.87, P = 0.0090; time F(4,12) = 24.90, P < 0.0001; interaction F(4, 12) = 14.04, P = 0.0002. Sidaks multiple comparisons test: 1h, P = 0.1276; 2h, *P = 0.0195; 3h, ***P = 0.0001; 4h, ****P < 0.0001.