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. Author manuscript; available in PMC: 2017 Jun 5.
Published in final edited form as: Nat Neurosci. 2016 Dec 5;20(1):20–23. doi: 10.1038/nn.4452

Figure 1. Targeting DREADDs to adult-born neurons in the OB.

Figure 1

(a) AAV2 vectors encoding cre-responsive DREADD-Gi and EGFP downstream of the human Synapsin promoter (pSYN; AAV2-Gi) were injected into the lateral ventricles of P42 Nestin-cre mice to target endogenous neural stem cells (P42-Gi mice). (b) Examples of EGFP+ cells in the P42-Gi OB at P70, and their colocalization with c-Fos after vehicle or CNO administration. Scale bars, 10 μm. (c) Dot plot of EGFP+/c-Fos+ double-labeled OB neurons calculated as percentages of total EGFP cells counted in vehicle- and CNO-administered P42-Gi mice. Significance (P<0.05, *) was tested by unpaired t test, percent double labeled, vehicle: 42.2% ± 5.5%, CNO: 20.1% ± 2.1%; t(4) = 3.72, P = 0.02.; n=100 EGFP+ cells/mouse, 3 mice/group. Data are mean ± s.e.m.