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. Author manuscript; available in PMC: 2017 Dec 10.
Published in final edited form as: J Control Release. 2016 Sep 26;243:69–77. doi: 10.1016/j.jconrel.2016.09.010

Figure 3.

Figure 3

MDA-MB-231 intracellular imaging, with top row (A–D) depicting 7 FR-targeted PCCAs activated intracellularly after insonation at MI = 1.0 (A) Calcein stains the cytoplasm of living cells. (B) DiI stains the lipid shell of PCCAs. (C) Transmitted light channel shows bubble-like structures intracellularly. (D) All three channels overlaid shows the colocalization of DiI+ / calcein- regions. (E) Graph depicts the proportion of MDA-MB-231 cells (mean +/− SE) with at least 1 intracellularly activated microbubble before (left, light gray) and after (right, dark gray) ultrasound application.