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. Author manuscript; available in PMC: 2017 Dec 10.
Published in final edited form as: J Control Release. 2016 Sep 26;243:69–77. doi: 10.1016/j.jconrel.2016.09.010

Figure 4.

Figure 4

MCF-7 intracellular imaging, with top row (A–D) depicting 3 intracellular FR-targeted PCCAs after insonation at MI = 1.5 (A) Cytoplasmic stain calcein shows 3 circular voids. (B) DiI stains the lipid shell of PCCAs. (C) Transmitted light channel shows 3 bubbles intracellularly. (D) All three channels overlaid shows the colocalization of DiI+ / calcein- bubble regions. (E) Graph depicts the proportion of MCF-7 cells (mean +/− SE) with at least 1 intracellularly activated microbubble before (left, light gray) and after (right, dark gray) ultrasound application.