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. Author manuscript; available in PMC: 2017 Dec 10.
Published in final edited form as: J Control Release. 2016 Oct 13;243:232–242. doi: 10.1016/j.jconrel.2016.10.012

Figure 2. Uptake and intracellular fate of free and liposomal Dox in NDL cells.

Figure 2

a) Confocal fluorescence images of NDL cells incubated with free Dox or liposomal drug (ASDox-LTSLs or CuDox-LTSLs). Cells were exposed to drug for 30 min at 4°C, washed and incubated with media for 15 min, 5 h and 24 h at 37°C (rows 1, 3-4). Fluorescence images of NDL cells treated with free Dox and co-stained with LysoTracker® Blue (red and green colors, respectively, row 2). Dox co-localized with LysoTracker® Blue appears yellow-orange. b) Time course of nuclear accumulation of drug for free Dox compared to liposomal Dox, presented as percent maximum nuclear fluorescence intensity of free Dox at 24 h. c) The total Dox associated with NDL cells after 30 min incubation with free or liposomal Dox at 4°C. Statistical analyses were performed using one-way ANOVA followed by a Tukey Post Hoc test. ***p < 0.001.