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. Author manuscript; available in PMC: 2017 Dec 10.
Published in final edited form as: J Control Release. 2016 Oct 13;243:232–242. doi: 10.1016/j.jconrel.2016.10.012

Figure 6. Cytotoxicity of Dox and released CuDox in NDL and vascular endothelial cells.

Figure 6

Cell viability following treatment with free Dox, liposomal CuDox loaded at intraliposomal pH 8.4 (CuDox-LTSLs) or loaded at varied intraliposomal pH (CuDox-LTSLs7.4 and CuDox-LTSLs8.8) and compared to released CuDox from the corresponding liposomal preparations described above. a) Viability of NDL cells at 24 h following treatment. Cell viability following all drug treatments was significantly lower than in the media-alone control (NT control). b) Viability of HUVECs 24 h after treatment. HUVECs were incubated in media (Media Only), free Dox, CuDox-LTSLs or released CuDox, exposed to body temperature for 20 min (37°C), 42°C for 20 min (42°C), or 42°C for 5 and 20 min prior to and after drug exposure, respectively (42°C+Pre HT). Cell viability was lower in all drug treated groups receiving the same hyperthermia protocol than in “Media Only” (p < 0.001). In (b), cell viability in all drug-treated groups was normalized to the corresponding “Media Only” control group. *p < 0.05, **p < 0.01, ***p < 0.001.