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. Author manuscript; available in PMC: 2018 Jan 5.
Published in final edited form as: Vaccine. 2016 Dec 3;35(2):329–336. doi: 10.1016/j.vaccine.2016.11.059

Figure 5. viSNE analysis reveals multi-dimensional phenotype of activated pTfh cells that are significantly more frequent in HD IIV recipients at Day 7.

Figure 5

(A) viSNE maps displaying live pTfh cells highlight expression of ICOS, CD38, and Ki-67 for day 0 and day 7 of HD IIV. Intensity scales indicate the range of expression for each marker. Maps display cells from the concatenated files of groups indicated above the plots. The red gate was drawn based on dual CD38 and ICOS expression. (B) Statistical analysis of the frequency of cells within the red gated population for all individuals. Wilcoxon signed rank test between day 0 and day 7 for paired samples; Mann-Whitney test for comparison between groups

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