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. 2004 Aug;16(8):2035–2047. doi: 10.1105/tpc.104.023937

Figure 4.

Figure 4.

Ala409 and Ala420 Are Essential for AtMAP65-1 Binding to Microtubules.

(A) Arabidopsis wild-type and ple-4 mutant allele.

(B) Nuclei in the roots of wild-type and ple-4 7-d-old seedlings visualized with the DNA-specific dye YO-PRO.

(C) The intron (thin line) and exon (thick line) map of AtMAP65-3/PLE, with the position of the A421V mutation in the ple4 allele indicated. aa, amino acids.

(D) Alignment of the most conserved region of all AtMAP65 proteins. The corresponding sequences encoded by the ple4 allele and AtMAP65-1/A409D and AtMAP65-1/A420V mutant proteins are also shown.

(E) Microtubule cosedimentation assay using AtMAP65-1 and AtMAP65-1/A409D and A420V mutants. Supernatants (S) and pellets (P) were separated on an SDS-PAGE gel and stained with Coomassie.