Figure 3.

OEP14-His₆ Could Insert into Purified OMVs.

(A) Purity of the isolated OMVs. Total proteins of chloroplasts (chlpt, 9 μg) and isolated OMVs (2.5 μg) were resolved by SDS-PAGE. Immunobloting was performed using antibodies against pea Toc75 and Tic110.

(B) Import of OEP14-His₆ into isolated OMVs. Lane 1, [³⁵S]OEP14-His₆ used in the import reactions. Lane 2, OEP14-His₆ associated with the OMVs. Lane 3, same as lane 2 except the OMVs were further treated with thermolysin (thermolysin-post) to reveal the amount of OEP14-His₆ inserted into the vesicles. Molecular masses of marker proteins are labeled at left.

(C) Efficiencies of OEP14 import into chloroplasts and isolated OMVs. In vitro–translated OEP14 was imported into isolated chloroplasts or OMVs. The amounts of OEP14 associated or inserted were quantified and plotted as percentage of total [³⁵S]OEP14-His₆ added to the import reactions.