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. Author manuscript; available in PMC: 2017 Jan 20.
Published in final edited form as: Oncogene. 2016 Jun 27;36(3):397–409. doi: 10.1038/onc.2016.211

Figure 6. HIF-1α directly associates with p53.

Figure 6

(A) Total lysates of indicated cells (1 mg each) were immunoprecipitated (IP) by anti-p53 antibody (goat) or anti-Myc antibody and immunoblotted with anti-HIF-1α and anti-p53 antibody (mouse) as indicated. Lysates without antibody and goat IgG were used as a negative and positive controls, respectively. (B) Association of p53 and HIF-1α proteins were further confirmed by immunoprecipitation with antibody against HIF-1α and immunobloted with indicated antibodies. Rabbit IgG and lysates with no antibody were used as controls. (C–D) 3% input of total cell lysates of indicated cell lines were subject to immunoblot using HIF-1α, p53 and GAPDH antibodies as indicated.