Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Aug;16(8):2164–2175. doi: 10.1105/tpc.104.023515

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, American Society of Plant Biologists

PMC Copyright notice

Figure 3. — Inactivation of the psbP and psbQ Genes in Synechocystis 6803.

(A) Restriction maps of the constructs for creating ΔpsbP (sll1418, top) and ΔpsbQ (sll1638, bottom) mutants. Restriction sites used are HincII (H) and AvaI (A). White arrows indicate the coding region for the disrupted gene and its closest neighboring open reading frame. Antibiotic resistance cassettes are for kanamycin (Km^r) and spectinomycin (Sm^r/Sp^r).

(B) PCR segregation analysis of the ΔpsbP (left panel) and ΔpsbQ (right panel) mutants. Genomic DNA from wild-type (W), ΔpsbP (ΔP), or ΔpsbQ (ΔQ) strains was the template for amplification with gene-specific primers to confirm segregation of the respective mutations.