Figure 2.

Recombinant Src and Axl kinase substrate specificity. Microarrays were incubated with 125 ng ml⁻¹ of recombinant Src or 500 ng ml⁻¹ Axl kinase. (a) Raw images of array spot phosphorylation after 1 h demonstrate significant overlap in peptide phosphorylation. Some peptides were predominantly phosphorylated by either Src (row 3-column 11 and 5–6) or Axl (for example, spots 9–11 and 10–5). (b) Correlation plot of 143 peptide signal intensities showing relative substrate preference for Src and Axl kinase, indicated by the red and blue circle, respectively.