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. 2016 Dec 16;48(12):e279. doi: 10.1038/emm.2016.114

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Microarray reproducibility and linearity. Inter- and intra-chip correlations between peptide phosphorylation levels as assessed by Pearson's correlations (r) using 12 technical replicates of HCT116 lysate (7.5 μg) measured on 3 chips in a single run. (a) Linearity plot of two representative technical replicates. (b) CV plot of the technical replicas shown in (a). The threshold was set at 15% as recommended by the microarray manufacturer. (c) Linearity plot of biological replicates of 786-O cells in three independent experiments. (d) CV plot of the biological replicas shown in (c). Threshold at CV 15%. CV, coefficient of variation as determined by the ratio of the standard deviation to the mean signal intensity.