FIG 7.

Inhibition of LdeIF2α phosphorylation reduces the infection rate. (A) Uninfected PECs were used as a negative control. (B and C) Mouse PECs were isolated after stimulation with a 4% starch solution and infected with normal L. donovani promastigotes (UNS) (B) or with promastigotes preincubated with GSK2606414 for 3 h and washed in PBS prior to infection (C). (D) The infection rate was estimated by counting the infected macrophages per 100 macrophages after 48 h. Significant differences are indicated by * (P < 0.05).