FIG 4.

NMR analysis. (A) The majority of the pT58-P motif in the pT58-Myc peptide or the pT58-A motif in the pT58-P59A mutant peptide is in the trans isomer. (B) Comparison of two-dimensional (2D) ¹H-nuclear Overhauser effect spectroscopy (NOESY) NMR spectra of the wild-type T58 (left) and pT58P59 (middle) or pT58P59A (right) mutant peptides. Note that the intramolecular NOE peak annotations are indicated. The NOESY spectra were all collected with a mixing time of 300 ms on a 500-MHz Bruker NMR spectrometer at 288 K.