FIG 8.
EYA1 regulates Myc stability in breast cancer. (A) Western blot of cell lysates from passage 3 scramble shRNA- or shEya1-transfected MDA-MB-231 (left) and quantification (right). Protein levels in MDA-MB-231 cells transfected with scramble shRNA were set to 100%. P values were calculated using StatView t tests. (B) (Left) Graphic representation of cell numbers determined on the indicated days. The numbers were consistent in triplicate, and averages are shown. (Right) FACS analysis of cells at day 4. (C) Western blot of cells treated with CHX for different times. t1/2, half-life of cMyc. (D) Flow cytometry of scramble shRNA- or shEYA1-MDA-MB-231 cells released into medium from G2/M arrest by nocodazole and Western blot of cell lysates with anti-cyclin A and β-actin as a loading control. The error bars indicate SD.