Table 1.
Pathogenicity of Δmrb1 Strains and Complementation by pMGH1
| Inoculum | Inoculated Plants | Plants with Tumors/Percentagea |
|---|---|---|
| FB1Δmrb1 × FB2Δmrb1 (4/6)bc | 199 | 0/0 |
| FB1 × FB2 (4/1)b | 101 | 93/92 |
| FB1Δmrb1#12 × FB2 (1/1) | 20 | 20/100 |
| FB1Δmrb1#15 × FB2 (1/1) | 20 | 19/95 |
| FB1 × FB2Δmrb1#4 (1/1) | 15 | 7/47 |
| FB1 × FB2Δmrb1#9 (1/1) | 19 | 6/32 |
| FB1Δmrb1#15 × FB2Δmrb1#9 (1/1) | 15 | 0/0 |
| FB1Δmrb1#12 × FB2Δmrb1#4 (1/1) | 16 | 0/0 |
| FB1 × FB2 (1/1) | 20 | 19/95 |
| FB1Δmrb1/pMGH1 × FB2Δmrb1/pMGH1 (1/4) | 84 | 59/70 |
| FB1mrb1:eGFP × FB2mrb1:eGFP (1/1) | 14 | 8/57 |
| SG200Δmrb1 (2/5) | 138 | 82/59 |
| SG200 (2/1) | 65 | 43/66 |
Comparable results were obtained with Δmrb1 strains, in which the ip cassette was substituted by the hph cassette.
a
Number of plants possessing at least one tumor and indication of the respective percentage.
b
(x/y): x is the number of independent pathogenicity tests performed; y is the number of different strains or strain combinations.
c
Plants were inspected 6 to 9 DAI. Tumors of wild-type infected plants were already present 6 DAI.