Table 4.

Interaction among Mrb1, Lga2, and Rga2

Combination	pLexA Construct	pB42AD Construct	β-Galactosidase Activitya
1	pLexA-mrb142	pB42AD-mrb141	295.2 ± 78.3 (6/1)b
2	pLexA-mrb142	pB42AD-lga244	17.8 ± 11.2 (8/1)
3	pLexA-mrb142	pB42AD-rga222	1364.4 ± 140.2 (6/2)
4	pLexA-lga243	pB42AD-mrb141	8.4 ± 5.6 (8/1)
5	pLexA-lga243	pB42AD-lga244	6.6 ± 3.7 (9/1)
6	pLexA-lga243	pB42AD-rga222	10.8 ± 3.3 (8/1)
7	pLexA-rga222	pB42AD-mrb141	252.8 ± 47.8 (11/1)
8	pLexA-rga222	pB42AD-lga244	2.6 ± 2.4 (11/1)
9	pLexA-rga222	pB42AD-rga222	11.4 ± 2.2 (12/1)
10	pLexA-mrb142	–c	0.1 ± 0.2 (4/1)
11	pLexA-lga243	–	1.2 ± 0.5 (6/1)
12	pLexA-rga222	–	0.2 ± 0.3 (4/1)
13	–	pB42AD-mrb141	9.3 ± 5.2 (11/1)
14	–	pB42AD-lga244	5.3 ± 4.4 (5/1)
15	–	pB42AD-rga222	7.0 ± 1.4 (4/1)
16d	pGB-E1473	pW2546-GA	135.3 ± 21.1 (4/6)
17d	pGB-E2473	pW2546-GA	5.1 ± 3.3 (4/1)
18d	–	pW2546-GA	4.9 ± 2.2 (4/1)
19	PLexA	pB42AD	11.7 ± 3.7 (4/2)

^aβ-Galactosidase activity was assayed as described in Methods; β-galactosidase activity in Miller units ± sd.

^b(x/y): x is the number of independent transformants tested for each quantification; y is the number of repetitions performed with each series.

^cIn this combination, either the pB42AD or the pLexA construct was omitted.

^dAs positive control the combination pGB-E1₄₇₃/pW2₅₄₆-GA, containing coding regions for interacting domains of the bE1 and bW2 proteins fused to either the DNA binding or activation domain of GAL4 (Kämper et al., 1995), was used. As negative controls, the combination pGB-E2₄₇₃/pW2₅₄₆-GA, in which bE and bW gene fragments are derived from the same allele, and pW2₅₄₆-GA alone were included.