FIG 1.

Impact of pbp gene deletions on the growth of PA14. (A) Single and double deletions of the HMM PBPs PBP1a (ponA), PBP1b (mrcB), and PBP2 (pbpA) (the subscript TP indicates deletion of the transpeptidase domain only). (B) Deletion of PBP3x (pbpC) and conditional deletion of PBP3 (ftsI). (C) Deletion of the GTase domains of PBP1a and PBP1b (the subscript GT indicates deletion of the glycosyltransferase domain only). The P. aeruginosa strains were cultivated in LB broth (with arabinose or glucose, where indicated), and cell growth was monitored by measuring the optical density at 600 nm. wt, wild type.