FIG 2.

Cell morphology and cell length of pbp mutants. (A) The effect of HMM PBP deletion on cell morphology was determined by scanning electron microscopy. Cultures from early log phase were harvested and prepared as described in Materials and Methods. (B) Cell length of pbp mutants. More than 100 cells were counted and measured by the use of ImageJ software. Student's t test was performed for determination of the significance of the differences between the wild type and the pbp mutants. ***, P < 0.0001. The median average length is shown in parentheses.