TABLE 3.
Strain | Genotype | MICa (μg/ml) |
|||||||
---|---|---|---|---|---|---|---|---|---|
IMP | DOP | CEP | CESD | CAZ | PIP | PenG | ATM | ||
PA14 | Wild type | 1 | 0.25 | 2 | 4 | 4 | 4 | 2,048 | 8 |
CW29 | ΔponATP | 1 | 0.25 | 2 | 2 | 4 | 4 | 2,048 | 8 |
CW31 | ΔmrcBTP | 1 | 0.25 | 2 | 2 | 4 | 4 | 512 | 8 |
CW37 | ΔpbpA | 0.25 | 0.25 | 2 | 4 | 4 | 4 | 256 | 8 |
CW46b | ΔftsI | 0.25 | 0.125 | 0.25 | 0.5 | 0.5 | 1 | 64 | 1 |
CW30 | ΔponATP pbpA::Gm | 0.25 | 0.25 | 2 | 16 | 4 | 64 | 1,024 | 8 |
CW34 | ΔmrcBTP pbpA::Gm | 0.25 | 0.25 | 2 | 4 | 4 | 4 | 512 | 8 |
CW47b | ΔponATP rmrcBTP | 1 | 0.25 | 1 | 2 | 2 | 4 | 256 | 8 |
IMP, imipenem; DOP, doripenem; CEP, cefepime; CESD, cefsulodin; CAZ, ceftazidime; PIP, piperacillin; PenG, penicillin G; ATM, aztreonam.
Strains CW46 and CW47 were grown in LB supplemented with 0.2% glucose, which was used to repress ectopic expression of PBP3 and PBP1a, respectively.