FIG 1.

Effects of DAP on the cytoplasmic membrane and cell wall of the DAP^s CB1631 (A) or DAP^r CB1634 (B) bacterial strain. Bacteria were grown in TSB (with or without DAP) at 37°C to late exponential phase (2.5 h) and labeled for 5 min with FM1-43FX (membrane; top) or HADA (peptidoglycan insertion; bottom). A Nikon inverted epifluorescence microscope was used. Exposure and contrast settings were optimized for each image; i.e., the brightness was not comparable between fields. Scale bars are 1 μm.