FIG 2.

Localization of PBP 2-GFP fusions in DAP^r cells treated with OXA, DAP, or DAP-OXA. (A) The DAP^r CB1634 strain producing PBP 2-GFP was grown with or without sublethal concentrations of DAP-OXA (D/O; 0.5× MIC), followed by labeling with Bodipy FL-VAN, fixation, and imaging by fluorescence microscopy. (B) DAP^r CB1634 cells producing PBP 2-GFP were induced with IPTG in the presence or absence of DAP, OXA, or the DAP-OXA combination, fixed, and imaged by fluorescence microscopy. Scale bars are 1 μm.