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Analysis of PBPs from CB1634 cells treated with OXA, DAP, or DAP-OXA. Membrane preparations obtained from CB1634 cells untreated or treated with OXA (0.5 μg/ml), DAP (1 μg/ml), or DAP-OXA (0.5 μg/ml and 1 μg/ml, respectively) were analyzed for the detection of penicillin-binding proteins 1 to 4. Equal amounts (20 μg) of Bocillin FL-labeled membrane proteins were separated by 10% SDS-PAGE. Arrows, fluorescently labeled PBPs.
