FIG 6.

Effect of mprF mutations on PrsA membrane localization. (A) Western blot analysis of the PrsA protein in membrane protein extracts (top) and extracellular protein extracts (bottom) from DAP^s CB1631, DAP^r CB1634, CB1643 ΔmprF, CB1634 ΔmprF mprF (WT), and CB1634 ΔmprF mprFL826F cells grown without antibiotics. Carbonic anhydrase was used a loading control. (B) Western blot analysis for PBP 2a and PrsA in membrane extracts from DAP^r CB1634 cells grown without (control [C]) or with OXA, DAP, or the DAP-OXA combination.