TABLE 2.

Strains and primers used in this study

Strain or primer	Description or sequence	Reference or source
S. aureus strains
CB5011	Daptomycin susceptible	6
CB5012	Daptomycin-resistant strain isogenic to CB5011, mprFL826F	6
CB5013	Daptomycin susceptible	6
CB5014	Daptomycin-resistant strain isogenic to CB5013, mprFS377L	6
CB1631	Daptomycin susceptible	6
CB1634	Daptomycin-resistant strain isogenic to CB1631, mprFL826F	6
MAR17	CB1634 ΔmprF::cat	6
MAR18	MAR-17/pMPRF-1 (wild type)	6
MAR19	MAR-17/pMPRF-2 (L826F mutant)	6
CB5013+VraSR	Entire vraS and vraR sequences cloned into pAW8	This study; 49
CB1631+VraSR	vraS and vraR sequences cloned into pAW8
Primers and probes
PrsA-F	AGTTAATGATAAGAAGATTGACGA
PrsA-R	GAAGGGCCTTTTCAAATTTATCTTT
VraSR-F	GGTGCAACGTTCCCATATTGTATTGT
VraSR-R	GGCTTCAACTCATGGGCTTTGGCAA
mprF-F	GGTGGCTTTATTGGTGCAGGCG
mprF-R	GATGCATCGAAAACATGGAA
mecA-F	TGCCTAATCTCATTGTGTTCCTGTAT
mecA-R	CGGTGCTGAAACTTTCACAATATAAT
pbp2-GFPF (DPH407)	GATAGCGGCCGCATGACGGAAAACAAAGGATCTTCTC
pbp2-GFPR (DPH408)	GAAGGGATCCTTAGTTGAATATACCTGTTAATCCACCG
16S-F	TCCGGAATTATTGGGCGTAA
16S-R	CCACTTTCCTCTTCTGCACTCA