TABLE 1.

Essential agreement between R1 and R2 of MIC Strip Isavuconazole test and between strip and EUCAST MICs

CYP51A profile	n	Strip MIC agreement (%)a between R1 and R2						Agreement (%)a between Strip MIC and EUCAST MIC
		R1 vs R2, FIE			R1 vs R2, 80% IE			R1 FIE vs EUCAST			R2 FIE vs EUCAST			R1 80% IE vs EUCAST			R2 80% IE vs EUCAST
		±0b	±1	±2	±0	±1	±2	±0	±1	±2	±0	±1	±2	±0	±1	±2	±0	±1	±2
Wild type	40	55	95	100	83	98	98	38	80	98	28	78	95	18	63	88	15	63	88
G54 alterations
G54E	4	100	100	100	75	100	100	75	100	100	75	100	100	0	100	100	0	75	100
G54R	1	100	100	100	100	100	100	0	0	100	0	0	100	0	0	0	0	0	0
G54V	1	0	100	100	100	100	100	0	100	100	100	100	100	0	100	100	0	100	100
G54W	3	100	100	100	67	100	100	33	100	100	33	100	100	67	100	100	100	100	100
G54R N248K	1	0	100	100	100	100	100	0	100	100	100	100	100	0	100	100	0	100	100
M220 alterations
M220K	3	33	100	100	67	100	100	0	100	100	67	100	100	0	100	100	0	67	100
M220T	2	50	100	100	50	100	100	100	100	100	50	100	100	0	50	100	50	50	100
M220V	2	50	100	100	50	50	100	0	50	100	50	50	100	0	50	100	0	50	50
M220I	2	50	100	100	50	50	100	50	50	50	0	50	50	0	50	100	50	100	100
M220I V101F	1	100	100	100	0	100	100	0	0	100	0	0	100	0	0	0	0	0	100
Mutants with TRc
TR34/L98H	9	100	100	100	33	78	89	11	11	33	11	11	33	11	22	100	33	67	100
TR46/Y121F T289A	10	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100	100
All isolates	79	68	97	100	75	94	97	42	75	90	41	73	89	25	66	91	30	70	91

^aPercent essential agreement within ±0, ±1, and ±2 twofold dilutions, respectively. MICs were read after 2 days of incubation. Strip MICs were read by using the FIE or 80% IE endpoint.

^bNumber of twofold dilutions.

^cTR, tandem repeat in the CYP51A promoter region.