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. 2016 Dec 22;7:13721. doi: 10.1038/ncomms13721

Figure 2. Activation of the mAON reduces olfactory sensitivity and impairs the performance of olfaction-dependent behaviours.

Figure 2

(a) AAV-mediated expression of hM3D-mCherry in CaMKIIa-positive neurons was restricted to the mAON. (b) CNO treatment did not alter investigation of mineral oil (0% odour concentration) across habituation trials (inset; data obtained from olfactory habituation/dishabituation test, Supplementary Fig. 4a), but impaired detection of an odour at a low concentration compared with vehicle treatment (n=12, two-way ANOVA both main effects F(2,22)>10.50, P<0.001). (c) CNO treatment increased the latency to locate a buried food reward (n=10, paired-samples t-test, t(9)=5.57 *P<0.001). (d) CNO-treated mice exhibited no change in sociability (vehicle group n=10, CNO group n=11, independent-samples t-test, t(19)=0.41, ns, P=0.68), but failed to distinguish between a novel and familiar conspecific (independent-samples t(19)=2.50, P<0.05; paired-samples t-tests, vehicle group t(9)=3.71, P<0.01, CNO group t(10)=0.60, ns, P=0.56; absolute investigation time for novel conspecific between groups, t(19)=1.13, ns, P=0.25). ANOVA, analysis of variance.