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. 2016 Dec 22;7:13874. doi: 10.1038/ncomms13874

Figure 3. Loss of p120 induces cytokinesis defects.

Figure 3

(a,b) Quantifications of the length of (a) early mitosis (pro-metaphase and metaphase) and (b) late mitosis/cytokinesis (anaphase and telophase) in PMC-1, control and dox-treated Trp53Δ/Δ-7 p120-iKD and U2OS p120-iKD cell lines. Statistical significance was determined using the Student’s t-test. *P<0.05, **P<0.01, ***P<0.001. (c) Time-lapse imaging of PMC-1, and control and dox-treated Trp53Δ/Δ-7 p120-iKD and U2OS p120-iKD cells. Shown are 10X DIC images. Scale bar, 25 μm. (d) High-magnification time-lapse imaging of H2B-mCherry expressing control and dox-treated U2OS p120-iKD. Shown are × 100 images. Arrowheads depict severe membrane deformation in dox-treated anaphase cells leading to cytokinesis failure. Scale bar, 10 μm. (e) Loss of p120 induces binucleation through cytokinesis defects during anaphase. During subsequent cell divisions, this can give rise to chromosomal instability and aneuploidy in cancer cells due to the presence of extra centrosomes.