Table 1. MsbA single-point mutations used for probing its catalytic centre.
Motif | Mutation | |
---|---|---|
Walker A (WA) | 376-GRSGSGKST-384 | K382A |
Q loop (Q) | 422-VSQNV-426 | Q424A |
Signature motif (S) | 481-LSGGQ-485 | S482A |
Walker B (WB) | 501-ILILDE-506 | D505A |
D loop (D) | 509-SALD-512 | D512A |
The bold entries indicate point of mutation.