Table 1.
Chromatographic conditions used in the TcNTPDase-1-ICER two-dimensional method.
| Pump | Time (min) | Valve position | Event |
|---|---|---|---|
| A | 0.0–0.3 | 1 | Enzymatic reaction in TcNTPDase-1-ICER |
| B | 0.0–0.5 | 1 | Analytical column conditioning |
| A | 0.31–8.0 | 2 | Analyte transfer to the analytical column |
| A | 8.01–20.0 | 1 | TcNTPDase-1-ICER conditioning |
| B | 8.01–20.0 | 1 | Analytical separation of the analytes in the second dimension |
Pump A: flow rate: 0.03 mL·min−1, eluent: Buffer A. Pump B: flow rate: 1.0 mL·min−1, eluent: Buffer C : MeOH (84 : 16; v/v).