Figure 1.

The treatment with ISCOMATRIX^TM adjuvant does not affect the exosome-associated fluorescence. Fluorescent exosomes were incubated in the absence or presence of 23 ISCO^TM U/mL of the adjuvant at 37 °C with aldehyde sulphate beads in a rotating plate. After 1 and 3 h, samples were washed, and analyzed by FACS. As a control, equivalent amounts of fluorescent exosomes were heated at 95 °C for 10 min before incubation with beads. Results representative of three independent experiments with duplicates are shown. In each plot, both percentages and, where relevant, mean fluorescence intensity (MFI) of fluorescent beads are indicated. At the bottom, mean values ± SD of both percentages and MFI of fluorescent beads from the three experiments are reported. Iscomatrix: ISCOMATRIX^TM adjuvant.