Figure 5.

Chloroquine inhibits ZIKV infection in human neural stem cells (hNSCs). hNSCs were infected with ZIKV MR766 at an MOI of 2 and incubated with increasing concentrations of chloroquine for 4 days. (A) The frequency of ZIKV-infected cells was analyzed by 4G2 staining and flow cytometry; (B) Chloroquine cytotoxicity was assessed by the viability of uninfected hNSCs treated with chloroquine; (C) Chloroquine treatment protection from ZIKV infection was evaluated by cell viability measurement at 4 days post-infection. Immunofluorescence NSCs infected with ZIKV MR766 (D) or ZIKV BR (E) at an MOI of 2 and treated with chloroquine for 4 days with 4G2 antibody (green) and DAPI (blue). Data are represented as mean ± SD from two to three independent experiments. Statistical analysis was performed with Kruskal–Wallis test and multiple comparisons with infected and untreated control corrected by Dunn’s test (* p < 0.05).