. 2016 Dec 14;5(4):53. doi: 10.3390/biology5040053

Table 2.

Michaelis constants for substrates (K_m) determined for the mitochondrial and nuclear GDH from rat liver [26].

Substrate	Measurement Conditions	Mitochondrial GDH	Nuclear GDH
Substrate	Measurement Conditions	K_m, mM	K_m, mM
Glutamate	50 mM K₃PO₄, pH 9.0, 0.05 mM NAD⁺	4.35	0.91
2-oxoglutarate	50 mM K₃PO₄, pH 7.6, 0.05 mM NADH, 50 mM NH₄Cl	0.45	0.13
NH₄⁺ (NH₄Cl)	50 mM K₃PO₄, pH 7.6, 0.05 mM NADH, 1.25 mM 2-oxoglutarate	30.0	11.0
NAD⁺	50 mM K₃PO₄, pH 9.0, 25 mM glutamate	0.02	0.06
NADH	50 mM K₃PO₄, pH 7.6, 50 mM NH₄Cl, 1.25 mM 2-oxoglutarate	0.02	0.02