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Comparison of global metabolite extraction efficiency: MDA-MB-231 cells were extracted using the “one-step in-plate”, ” two-step in-plate” or “in-tube” protocol (see Figure 1) and analysed on an LC-MS untargeted metabolomics platform. y-axis (relative abundance) represents the median peak height from all detected LC-MS peaks (mean ± SE; n = 3–4).
