Figure 4.

Precision and repeatability of the “One-step in-plate” method: The relative standard deviation (RSD) for each metabolite was determined for: (A) each of the extraction methods within a single experiment (n = 3–4); and (B) the “one-step in-plate” method performed on seven independent occasions (Note: experiments 2a and 2b were analysed in a single LC-MS batch, as were 5a and 5b). Box plots represent the median ± interquartile range, and whiskers show the 5th and 95th percentile (n = 3–4). **** p ≤ 0.0001, using one-way ANOVA with Tukey’s multiple comparison test.