Figure 2.

Time course for ²H-incorporation into DNA-derived deoxyribose and protein-derived alanine in C2C12 myoblasts following incubation in 4% ²H₂O over 120 h ²H₂O treatment. Excess molar enrichment in the M₁ isotopomer (EM₁) over time in DNA-derived deoxyribose and protein-derived alanine (A); the percent turnover of the cellular DNA and protein pools over time (B). Inset: fractional synthesis rate constant (k), half-life (t_1/2) and goodness-of-fit (R²) from non-linear curve fitting. Two replicates were performed for each time point. Error bars represent the standard error of the mean (SEM).