Figure 3.

Comparison of ²H-incorporation into DNA-derived deoxyribose and protein-derived alanine in C2C12 myoblasts and myotubes following incubation in 4% ²H₂O treatment over 96 h. Representative images of myoblast and differentiating myotubes (A); excess molar enrichment in the M₁ isotopomer (EM₁) over time in DNA-derived deoxyribose (B) and protein-derived alanine (C) in myoblasts and myotubes. Inset: fractional synthesis rate constant (k), half-life (t_1/2) and goodness-of-fit (R²) from non-linear curve fitting. Two replicates were performed for each time point. Error bars represent the standard error of the mean (SEM).