Table 1.

Statistically differentiating metabolites in melittin treated and untreated A2780 and A2780CR cells.

m/z	RT	Metabolite Name	S/R		MS/MR		MR/R		MS/S
			p-Value	Ratio	p-Value	Ratio	p-Value	Ratio	p-Value	Ratio
Proline/glutamate/arginine/polyamine metabolism
116.071	12.8	* Proline	<0.01	0.837	<0.001	0.409	ns	1.056	<0.001	0.516
128.035	10.3	1-Pyrroline-3-hydroxy-5-carboxylate	<0.05	0.839	<0.001	0.364	ns	1.114	<0.001	0.453
130.051	14.4	Glutamate-5-semialdehyde	ns	0.944	<0.001	0.607	ns	1.065	<0.001	0.658
131.083	11.4	* Ornithine	<0.001	4.774	<0.001	3.573	ns	1.001	<0.001	0.749
132.030	15.2	* Aspartate	ns	1.091	<0.001	1.229	ns	1.055	<0.01	1.159
146.046	10.8	Glutamate	<0.001	0.608	<0.001	0.113	ns	0.999	<0.001	0.187
147.076	14.9	* Glutamine	<0.001	0.299	<0.001	0.503	<0.001	1.204	<0.001	1.906
173.104	24.6	* Arginine	<0.001	0.155	<0.001	0.004	ns	1.023	<0.05	0.026
188.057	14.4	* N-Acetyl-l-glutamate	<0.001	0.637	<0.001	0.146	<0.001	1.348	<0.001	0.313
89.107	15.4	** Putrescine	<0.001	2.339	<0.001	1.490	ns	1.051	<0.001	0.670
131.118	8.24	N-Acetylputrescine	<0.001	5.175	<0.001	1.603	ns	1.021	<0.001	0.316
146.165	26.2	** Spermidine	<0.001	2.354	ns	1.150	ns	1.127	<0.001	0.550
150.058	11.4	* Methionine	<0.001	0.422	<0.001	0.669	<0.01	1.127	<0.001	1.594
298.096	6.42	* 5′-Methylthioadenosine	0.003	1.92	ns	0.830	ns	0.839	<0.001	0.361
TCA cycle/glycolysis
133.014	16.4	* Malate	<0.001	0.647	<0.001	0.238	<0.001	1.129	<0.001	0.418
145.014	15.9	* 2-Oxoglutarate	ns	0.972	<0.001	0.173	<0.001	1.217	<0.001	0.221
191.020	18.4	* Citrate	<0.001	2.207	<0.001	0.534	ns	1.098	<0.001	0.265
508.003	16.6	* ATP	<0.001	0.267	<0.001	0.118	ns	0.963	<0.001	0.415
Carnitine metabolism/fatty acid metabolism
162.112	13.3	* Carnitine	<0.001	0.253	<0.001	0.164	ns	1.065	<0.001	0.676
204.123	11.0	* Acetylcarnitine	<0.001	0.273	<0.001	0.050	ns	1.016	<0.001	0.190
232.154	8.7	Butanoylcarnitine	<0.001	14.083	ns	1.992	ns	0.903	<0.001	0.131
664.117	14.3	* NAD+	<0.001	0.487	<0.001	0.135	<0.001	1.228	<0.001	0.336
Miscellaneous
104.106	19.6	* Choline	<0.001	0.019	<0.001	0.231	<0.001	1.423	<0.001	7.270
166.086	10.0	* Phenylalanine	<0.001	0.381	<0.05	0.873	<0.01	1.157	<0.001	2.358
118.086	12.4	* Valine	<0.001	1.148	<0.01	2.257	<0.01	1.148	<0.001	2.257
120.065	14.3	* Threonine	<0.001	0.610	ns	0.948	<0.05	1.140	<0.001	1.704
88.040	14.7	* Alanine	ns	0.965	<0.001	0.385	ns	1.098	<0.001	0.442
179.056	17.1	* Hexose	<0.001	0.564	<0.001	0.364	<0.01	0.931	<0.001	0.605
195.051	13.7	* Gluconic acid	<0.001	0.559	<0.001	0.103	<0.001	0.837	<0.001	0.154
258.110	14.4	* Glycerophosphocholine	<0.001	0.020	<0.001	0.031	<0.001	1.529	<0.001	2.342

RT: retention time (in min); MR: melittin treated A2780CR cells; R: untreated A2780CR cells; MS: melittin treated A2780 cells; S: untreated A2780 cells; ns: non-significant. * Retention time matches standard on ZIC-pHILIC column; ** Retention time matches standard on ZIC-HILIC column.