Table 5.
Cloning and expression hosts and plasmids used in this study.
| Strain/Plasmid | Details | Reference |
|---|---|---|
| Escherichia coli Top10 | Cloning host | Invitrogen, C404010, Paisley, United Kingdom |
| Escherichia coli BL21 (DE3) | Expression host | Novagen, Madison, WI, USA |
| pBAD_Myc_HisA | Cloning and expression vector | Thermo Scientific, V44001, Waltham, MA, USA |
| PG_ChiA | pBAD_Myc_HisA vector containing the EA58_02560 gene | This study |
| PG_ChiD | pBAD_Myc_HisA vector containing the EA58_19900 gene | This study |
| PG_GH19 | pBAD_Myc_HisA vector containing the EA58_12180 gene | This study |
| PP_ChiA | pBAD_Myc_HisA vector containing the TW73_17595 gene | This study |
| PP_ChiD | pBAD_Myc_HisA vector containing the TW73_14030 gene | This study |
| PP_GH18 | pBAD_Myc_HisA vector containing the TW73_13265 gene | This study |
| PP_GH19 | pBAD_Myc_HisA vector containing the TW73_14810 gene | This study |